Journal: Thoracic Cancer

Article Title: S‐ and R‐Carvedilol Prevent Benzo(a)pyrene‐Induced Lung Carcinogenesis

doi: 10.1111/1759-7714.70109

Figure Lengend Snippet: Effects of S‐ and R‐carvedilol on BPDE‐induced lung epithelial cell transformation: Benzo(a)pyrene diol epoxide (BPDE), an active metabolite of B(a)P, was used to transform BEAS‐2B cells. (A) & (B) The cells were pre‐treated with 5 μM S‐ and R‐carvedilol, 5 μM atenolol, 5 μM propranolol (Prop), and 5 μM ICI‐118551 (ICI) for 2 h, and then treated with 0.2 μM of BPDE for 1 h. The cells were then cultured with 5 μM of the drugs for 7 days. Afterward, they were seeded in soft agar in a 96‐well plate (2000 cells/well) containing 5 μM of the drugs in the top layer of agar. The cell colonies were counted under a microscope after 10 days of incubation. (C) Representative images were taken using GelCount of the wells containing colonies that grew on agar after 10 days of incubation. The plotted data are represented as mean ± SD, n = 6 to 8. The one‐way ANOVA followed by Dunnett's multiple comparison test was used to assess statistical differences. Statistical significance was denoted as ****: P < 0.0001 and **: P < 0.01.

Article Snippet: The BEAS‐2B cell line, originating from normal human bronchial epithelial cells, was obtained from ATCC (catalog number CRL‐9609).

Techniques: Transformation Assay, Cell Culture, Microscopy, Incubation, Comparison

Journal: Oncotarget

Article Title: Cancer cell-selective, clathrin-mediated endocytosis of aptamer decorated nanoparticles

doi: 10.18632/oncotarget.24772

Figure Lengend Snippet: Cells were incubated with 50 nM S15-APT QDs for 2 h. Nuclei were stained with 2 μg/ml Hoechst 33342. Fluorescence confocal microscopy was performed using an inverted confocal microscope (Zeiss LSM 710) at ×630 magnification. ( A ) Human A549 non-small cell lung carcinoma cells; ( B ) Normal human bronchial epithelial BEAS2B cells which served as normal non-target cells, ( C ) Human colon adenocarcinoma CaCo-2 cells; ( D ) Human cervical carcinoma HeLa cells; ( E ) A549 cells were incubated with 50 nM S15-APT QDs along with 5 μM free APT; ( F ) A549 cells were incubated with 50 nM random sequence APT-QDs; ( G ) A549 cells incubated with no S15-APT QDs; H) A549 cells incubated with 50 nM Qdot ® 655; ( I ) ABCG2-overexpressing MDR subline A549/K1.5 cells incubated with 50 nM S15-APT QDs; the red fluorescence channel was defined between 10-100 for all presented images.

Article Snippet: Normal human bronchial epithelial BEAS2B cells were generously provided by Prof. Rotem Karni (The Hebrew University, Jerusalem, Israel).

Techniques: Incubation, Staining, Fluorescence, Confocal Microscopy, Microscopy, Sequencing

Journal: Neoplasia (New York, N.Y.)

Article Title: Multimutated Herpes Simplex Virus G207 Is a Potent Inhibitor of Angiogenesis 1

doi:

Figure Lengend Snippet: Susceptability of human endothelial cells (HUVEC and HDMEC), fibroblasts (HFF), retinal glial cells (HRG), normal human bronchial epithelial cells (NHBE), and alveolar rhabdomyosarcoma (KFR) cell cultures to G207 and HSV-1 wild-type strain McIntyre. Cells were infected with G207 or McIntyre at an MOI of 0.1. Each data point (mean of triplicate wells ± SD) is the percentage of surviving cells compared with mock-infected cells in control wells or infectious viral titers at each time point, respectively. Some error bars for data points are smaller than the symbol size.

Article Snippet: Normal human bronchial epithelial cells (NHBE) were obtained from Clonetics (CellSystems, St. Katharinen, Germany) and cultured in BEGM medium according to the instructions of the producer.

Techniques: Infection, Control